Determination of nicotine in wild edible fungi by QuEChERS-ultra performance liquid chromatography-triple quadrupole mass spectrometry

doi:10.3724/SP.J.1123.2018.11005

Abstract

Abstract:

Yunnan Province has many wild edible fungi resources, and of these, endogenous nicotine has received extensive attention in recent years. In this study, wild edible fungus was used as the research object, and the QuEChERS method was improved, including optimization of solvent extraction and purification conditions and optimization of the chromatographic behavior of nicotine under different mobile phase conditions for ultra performance liquid chromatography (UPLC). Combined with triple quadrupole mass spectrometry, a high-efficiency, rapid, and sensitive method for the determination of nicotine in wild edible fungi was established. The results showed that an ammonia:acetonitrile (6:94, v/v) mixed solution can completely extract nicotine from wild edible fungi, and the extraction solution was purified by graphitized carbon black (GCB) and N-propylethylenediamine (PSA) mixed filler. Then, a 0.1% (volume percentage) ammonia solution and acetonitrile were used as the mobile phases. The nicotine peak obtained in the positive ion multiple reaction monitoring (MRM) mode had a better peak shape and better response. The linear relationship of nicotine mass concentration with peak area in the range of 0.05-50.0 μg/L was good. The correlation coefficient (r²) was 0.9999. The limit of quantification was 0.2 μg/kg, and the limit of detection was 0.05 μg/kg. The average recovery rates at three spiked concentrations were in the range of 86.34%-96.4%, and the relative standard deviations varied from 4.44% to 6.3%. The sensitivity and recovery of this method are consistent with the rapid determination of nicotine in the edible fungus industry.

Key words: nicotine, triple quadrupole mass spectrometry, ultra performance liquid chromatography (UPLC), wild edible fungus

CLC Number:

O658

LIN Tao, WEI Maoqiong, YU Jidong, LI Maoxuan, ZOU Yanhong, SHA Lingjie, LIU Hongcheng. Determination of nicotine in wild edible fungi by QuEChERS-ultra performance liquid chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, 2019, 37(5): 512-517.

References

[1] Edible Fungus Branch, China Chamber of Commerce for Import and Export of Food, Livestock and Animals. Edible Fungi of Zhejiang, 2009, 17(3):17 中国食品土畜进出口商会食用菌分会. 浙江食用菌, 2009, 17(3):17
[2] Peng Y X, Wang Y G, Che Y Q, et al. Edible Fungi of Zhejiang, 2009, 17(3):19 彭云霞, 王裔耿, 车玥泉, 等. 浙江食用菌, 2009, 17(3):19
[3] EU Urgently Revised the Nicotine Limit Standard for Edible Fungi. (2009-05-11)[2019-03-02]. http://jckspaqj.aqsiq.gov.cn/xw/200905/t20090515_115574.htm
[4] Food Partner Network, Food Database, Japan Positive List Query.[2019-03-02]. http://db.foodmate.net/kending/agrdtl.php?a_inq=48300
[5] Nides M A, Leischow S J, Bhatter M, et al. Am J Health Behavior, 2014, 38(2):265
[6] Xu T Y, Lan X H, Guan Y F, et al. Acta Pharmacol Sinica, 2015, 36(4):429
[7] Hirata N, Yamada S, Asanagi M, et al. Biochem Biophys Res Commun, 2016, 470(2):300
[8] Chen K F, Huang S Y, Chung Y T, et al. Chem Eng J, 2018, 331:636
[9] Cavalieri C, Bolzoni L, Bandini M. Food Addit Contam, 2010, 27(4):473
[10] Lin X, Sun Y, Xu D, et al. Electrophoresis, 2013, 34:2033
[11] Müller C, Bracher F, Plössl F. Chromatography, 2011, 73(7/8):807
[12] Wang H, Zhao Q, Song W, et al. Talanta, 2011, 85(1):743
[13] Yang J, Wang W H, Wang Q, et al. Chinese Journal of Analysis Laboratory, 2011, 30(1):80 杨俊, 王文辉, 王齐, 等. 分析试验室, 2011, 30(1):80
[14] Li F Q, Shi L H, Wang F, et al. Chinese Journal of Chromatography, 2017, 35(6):620 李福琴, 石丽红, 王飞, 等. 色谱, 2017, 35(6):620
[15] Munaretto J S, Viera M D S, Martins M L, et al. J AOAC Int, 2016, 99(6):1426
[16] Song S, Zhang Z, Zou N, et al. Food Anal Methods, 2017, 10(12):3972
[17] Costa M E H, Collins C H, Jardim I C S F. Food Chem, 2018, 249:77
[18] Wang Y, Zhou W H, Jin Y M, et al. Journal of Instrumental Analysis, 2012, 31(7):797 王晔, 周宛虹, 金永明, 等. 分析测试学报, 2012, 31(7):797
[19] Li M X, Wang Q, Zhu J, et al. Chinese Journal of Chromatography, 2017, 35(8):826 李明昕, 王倩, 朱婧, 等. 色谱, 2017, 35(8):826
[20] Loukotková L, Von Tungeln L S, Vanlandingham M, et al. J Chromatogr B, 2018, 1072:229
[21] Abdallah I A, Hammell D C, Stinchcomb A L, et al. J Chromatogr B, 2016, 1020:67
[22] Tomizawa M, Casida J E. Annu Rev Pharmacol Toxicol, 2005, 45(45):247
[23] Quick Method for the Analysis of Numerous Highly Polar Pesticides in Foods of Plant Origin via LC-MS/MS Involving Simultaneous Extraction with Methanol (QuPPe-Method).[2019-03-02]. http://www.crl-pesticides.eu/library/docs/srm/meth_QuPPe.pdf
[24] Tambwekar K R, Kakariya R B, Garg S. J Pharm Biomed Anal, 2003, 32(3):441