Advancesin enrichment and detection methods for N-phosphorylated proteins

doi:10.3724/SP.J.1123.2019.09015

Abstract

Abstract:

Protein phosphorylation plays an important role in various life processes, such as signaling, metabolism, and growth. In addition to the well-known O-phosphorylation, which occurs on serine, threonine and tyrosine side chains, N-phosphorylation, occurring on histidine, arginine and lysine side chains, has attracted much attention. However, due to the chemical instability of N-phosphorylation, the studies on it are still in the nascent stage. In this review, enrichment and detection methods for protein N-phosphorylation are reviewed, and future research in this field is prospected.

Key words: protein N-phosphorylation, enrichment, detection, review

HU Yechen, JIANG Bo, ZHANG Lihua, ZHANG Yukui. Advancesin enrichment and detection methods for N-phosphorylated proteins[J]. Chinese Journal of Chromatography, 2020, 38(3): 278-286.

Figures/Tables 6

Fig. 1 Chemical structures of protein N-phosphorylation

Fig. 2 (a)Analogues of pHis and (b) structures of the three synthetic peptide libraries used in which either His or a stable pHis mimetic (6 or 7) is flanked by randomized, neutral amino acids (alanine and glycine)

Fig. 3 Flow chart of the enrichment and identification of pHis peptides[41] CID: collision induced dissociation.

Fig. 4 Strategy to develop anti-pArg antibodies[45] KLH: keyhole limpet hemocyanin.

Fig. 5 (a) Flowchart of retention time difference combining dimethyl labeling (ReDD) strategy and (b) chemical process of pLys peptide[52] FA: formic acid; SCX: strong cation exchange chromatography.

Fig. 6 (a) Flowchart of cleavable hydrophobic derivatization (CHD) strategy and (b) chemical process of pLys peptide[53] Dec-disulf-NHS: 2, 5-dioxopyrrolidin-1-yl-3-(decyldisulfanyl)propanoate; TCEP: tris(2-carboxyethyl)phosphine.

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