Chinese Journal of Chromatography ›› 2020, Vol. 38 ›› Issue (6): 627-638.DOI: 10.3724/SP.J.1123.2019.12007

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Advances in development and application of liquid chromatography coupled with isotope ratio mass spectrometry

DING Bo1,2,*(), XIE Jianjun2, WANG Zhiyuan2, TAO Yiwen1, HUANG Hongbo1, CHEN Wenrui2, YAO Xiaobao3,4   

  1. 1 School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou 511436, China
    2 Guangzhou Customs Technology Center, Guangzhou 510623, China
    3 Qinghai Province Association of Ophiocordyceps sinensis, Xining 810007, China
    4 Qinghai Gongcao Biotechnology Co. Ltd., Xining 810007, China
  • Received:2019-12-03 Online:2020-06-08 Published:2020-12-10
  • Contact: DING Bo
  • Supported by:
    Science & Technology Plan Project of Guangdong Province of China(2016A040403091);Science & Technology Plan Project of Guangzhou(201904010119)

Abstract:

The first commercial interface for hyphenating liquid chromatography with isotope ratio mass spectrometry (LC-IRMS) was not available until 2004, and it was commercialized under the name LC IsoLink by Thermo Finnigan. LC-IRMS, a method of compound-specific isotope analysis, has enabled the detection of stable carbon isotope ratios (δ13C) in targeted substances in order to determine their origin and authenticity. This paper provides an overview of IRMS and LC-IRMS techniques, the history and detailed classification of the latter over the past twenty years. The application of LC-IRMS to the fields of food safety, environment and ecology, life sciences, and archeology are reviewed. Finally, the technical limitations, current challenges, and future development trends of LC-IRMS are outlined.

Key words: isotope ratio mass spectrometry (IRMS), liquid chromatography (LC), compound-specific isotope analysis, δ13C, review