Advances in separation techniques for exosomes and their clinical applications

doi:10.3724/SP.J.1123.2019.02007

Abstract

Abstract: Exosomes are vesicles secreted by many types of cells through exocytosis, and their sizes range from 30 to 200 nm. Exosomes consist of a lipid bilayer membrane, containing a number of bioactive molecules, e. g., proteins, ribose nucleic acid (RNA), and deoxyribo nucleic acid (DNA) derived from the cell of origin. As intercellular communication carriers, exosomes participate in many physiological and pathological processes. Because of the complexity of body fluids, as well as the small size and low density of exosomes, the isolation of exosomes is an essential and challenging step before subsequent analysis and functional studies. This review summarizes the advances in the analytical approaches, characterization methods, biological functions and clinical applications of exosomes, with particular emphasis on exosomes isolation techniques.

Key words: exosome, clinical application, isolation method, review

CLC Number:

O658

GAO Fangyuan, JIAO Fenglong, ZHANG Yangjun, QIN Weijie, QIAN Xiaohong. Advances in separation techniques for exosomes and their clinical applications[J]. Chinese Journal of Chromatography, 2019, 37(10): 1071-1083.

References

[1] Pan B T, Johnstone R M. Cell, 1983, 33(3):967
[2] Johnstone R M, Adam M, Hammond J R, et al. J Biol Chem, 1987, 262(19):9412
[3] Keller S, Sanderson M P, Stoeck A, et al. Immunol Lett, 2006, 107(2):102
[4] Suchorska W M, Lach M S. Oncol Rep, 2016, 35(3):1237
[5] Shao H, Im H, Castro C M, et al. Chem Rev, 2018, 118(4):1917
[6] Llorente A, Skotland T, Sylvänne T, et al. BBA-Mol Cell Biol L, 2013, 1831(7):1302
[7] Purushothaman A, Bandari S K, Liu J, et al. J Biol Chem, 2016, 291(4):1652
[8] Urbanelli L, Magini A, Buratta S, et al. Genes, 2013, 4(2):152
[9] Meldolesi J. Curr Biol, 2018, 28(8):435
[10] Valadi H, Ekström K, Bossios A, et al. Nat cell biol, 2007, 9(6):654
[11] Vlassov A, Magdaleno S, Setterquist R, et al. BBA-Gen Subjects, 2012, 1820(7):940
[12] Stoorvogel W, Kleijmeer M J, Geuze H J, et al. Traffic, 2002, 3(5):321
[13] Vaidyanathan R, Naghibosadat M, Rauf S, et al. Anal Chem, 2014, 86(22):11125
[14] WangY Q, Sui Z G, Zhang L H, et al. Chinese Journal of Chromatography, 2016, 34(12):1131 翁叶靖, 随志刚, 张丽华, 等. 色谱, 2016, 34(12):1131
[15] Choi D S, Kim D K, Kim Y K, et al. Mass Spectrom Rev, 2015, 34(4):474
[16] Zabeo D, Cvjetkovic A, Lässer C, et al. J Extracell Vesicles, 2017, 6(1):1329476
[17] Yang J S, Lee J C, Byeon S K, et al. Anal Chem, 2017, 89(4):2488
[18] Bobrie A, Théry C. Biochem Soc Trans, 2013, 41(1):263
[19] Raposo G, Nijman H W, Stoorvogel W, et al. J Exp Med, 1996, 183(3):1161
[20] Van Niel G, Raposo G, Candalh C, et al. Gastroentero-logy, 2001, 121(2):337
[21] Momen-Heravi F, Balaj L, Alian S, et al. Front Physiol, 2012, 3:162
[22] An M, Lohse I, Tan Z, et al. J Proteome Res, 2017, 16(4):1763
[23] Kalra H, Adda C G, Liem M, et al. Proteomics, 2013, 13(22):3354
[24] Keller S, Ridinger J, Rupp A-K, et al. J Transl Med, 2011, 9(1):86
[25] Welton J L, Webber J P, Botos L-A, et al. J Extracell Vesicles, 2015, 4(1):27269
[26] Coumans F A, Brisson A R, Buzas E I, et al. Circ Res, 2017, 120(10):1632
[27] Cheruvanky A, Zhou H, Pisitkun T, et al. Am J Physiol-Renal, 2007, 292(5):F1657
[28] Abramowicz A, Widlak P, Pietrowska M. Mol BioSyst, 2016, 12(5):1407
[29] Zhao H, Yang L, Baddour J, et al. eLife, 2016, 5:e10250
[30] Tauro B J, Greening D W, Mathias R A, et al. Methods, 2012, 56(2):293
[31] Mathivanan S, Lim J W E, Tauro B J, et al. Mol Cell Proteomics, 2010, 9(2):197
[32] Koga K, Matsumoto K, Akiyoshi T, et al. Anticancer Res, 2005, 25(6A):3703
[33] Mizutani K, Terazawa R, Kameyama K, et al. Anticancer Res, 2014, 34(7):3419
[34] Echevarria J, Royo F, Pazos R, et al. Chembiochem, 2014, 15(11):1621
[35] Nakai W, Yoshida T, Diez D, et al. Sci Rep, 2016, 6:33935
[36] Kim J, Shin H, Kim J, et al. PloS One, 2015, 10(6):e0129760
[37] Zarovni N, Corrado A, Guazzi P, et al. Methods, 2015, 87:46
[38] Ko J, Bhagwat N, Black T, et al. Cancer Res, 2018, 78(13):3688
[39] Shin S, Han D, Park M C, et al. Sci Rep, 2017, 7(1):9907
[40] Woo H-K, Sunkara V, Park J, et al. ACS Nano, 2017, 11(2):1360
[41] Davies R T, Kim J, Jang S C, et al. Lab Chip, 2012, 12(24):5202
[42] Yasui T, Yanagida T, Ito S, et al. Sci Adv, 2017, 3(12):e1701133
[43] Lewis J M, Vyas A D, Qiu Y, et al. ACS Nano, 2018, 12(4):3311
[44] Ibsen S D, Wright J, Lewis J M, et al. ACS Nano, 2017, 11(7):6641
[45] Kang Y-T, Kim Y J, Bu J, et al. Nanoscale, 2017, 9(36):13495
[46] Reátegui E, Vos K E, Lai C P, et al. Nat Commun, 2018, 9(1):175
[47] Liu C, Guo J, Tian F, et al. ACS Nano, 2017, 11(7):6968
[48] Wu M, Ouyang Y, Wang Z, et al. Proc Natl Acad Sci U S A, 2017, 114(40):10584
[49] Wu X, Li L, Iliuk A, et al. J Proteome Res, 2018, 17(9):3308
[50] Wan Y, Cheng G, Liu X, et al. Nat Biomed Eng, 2017, 1(4):0058
[51] Gao F, Jiao F, Xia C, et al. Chem Sci, 2019, 10:1565
[52] Li P, Kaslan M, Lee S H, et al. Theranostics, 2017, 7(3):789
[53] Pisitkun T, Shen R F, Knepper M A. Proc Natl Acad Sci U S A, 2004, 101(36):13368
[54] Melo S A, Luecke L B, Kahlert C, et al. Nature, 2015, 523(7559):177
[55] Van Der Pol E, Hoekstra A, Sturk A, et al. J Thromb Haemostasis, 2010, 8(12):2596
[56] Tang Y-T, Huang Y-Y, Zheng L, et al. Int J Mol Med, 2017, 40(3):834
[57] Théry C, Amigorena S, Raposo G, et al. Curr Protoc Cell Biol, 2006:3.22.1
[58] Comelli L, Rocchiccioli S, Smirni S, et al. Mol Bio Syst, 2014, 10(5):1146
[59] Tian Y, Ma L, Gong M, et al. ACS Nano, 2018, 12(1):671
[60] Mathivanan S, Ji H, Simpson R J. J Proteomics, 2010, 73(10):1907
[61] Barry O P, Praticò D, Savani R C, et al. J Clin Invest, 1998, 102(1):136
[62] Yáñez-Mó M, Siljander P R-M, Andreu Z, et al. J Extracell Vesicles, 2015, 4(1):27066
[63] He M, Qin H, Poon T C, et al. Carcinogenesis, 2015, 36(9):1008
[64] Iero M, Valenti R, Huber V, et al. Cell Death Differ, 2008, 15(1):80
[65] Zomer A, Maynard C, Verweij F, et al. Cell, 2015, 161(5):1046
[66] Hoshino A, Costa-Silva B, Shen T L, et al. Nature, 2015, 527(7578):329
[67] Costa-Silva B, Aiello N M, Ocean A J, et al. Nat Cell Biol, 2015, 17(6):816
[68] Peinado H, Aleckovic M, Lavotshkin S, et al. Nat Med, 2012, 18(6):883
[69] Maji S, Chaudhary P, Akopova I, et al. Mol Cancer Res, 2017, 15(1):93
[70] Qu L, Ding J, Chen C, et al. Cancer Cell, 2016, 29(5):653
[71] Yeung C L A, Co N-N, Tsuruga T, et al. Nat Commun, 2016, 7:11150
[72] Mikamori M, Yamada D, Eguchi H, et al. Sci Rep, 2017, 7:42339
[73] Qin X, Yu S, Xu X, et al. Oncotarget, 2017, 8(26):42125
[74] Chen G, Huang A C, Zhang W, et al. Nature, 2018, 560:382
[75] Chalmin F, Ladoire S, Mignot G, et al. J Clin Invest, 2010, 120(2):457
[76] Wang X, Luo G, Zhang K, et al. Cancer Res, 2018, 78(16):4586
[77] Arscott W T, Tandle A T, Zhao S, et al. Transl Oncol, 2013, 6(6):638
[78] Kumar Jella K, Rani S, O'driscoll L, et al. Radiat Res, 2014, 181(2):138
[79] Record M, Subra C, Silvente-Poirot S, et al. Biochem Pharmacol, 2011, 81(10):1171
[80] Zitvogel L, Regnault A, Lozier A, et al. Nat Med, 1998, 4:594
[81] Munich S, Sobovujanovic A, Buchser W J, et al. Oncoimmunology, 2012, 1(7):1074
[82] Sanchita B, Schorey J S. J Biol Chem, 2007, 282(35):25779
[83] Skokos D, Panse S, Le, Villa I, et al. J Immunol, 2001, 166(2):868
[84] Vlist E J V D, Arkesteijn G J A, Lest C H a V D, et al. J Extracell Vesicles, 2012, 1(1):18364
[85] Arslan F, Lai R C, Smeets M B, et al. Stem Cell Res, 2013, 10(3):301
[86] Bruno S, Collino F, Deregibus M C, et al. Stem Cells Dev, 2012, 22(5):758
[87] Rahman M J, Regn D, Bashratyan R, et al. Diabetes, 2014, 63(3):1008
[88] Zhu W, Huang L, Li Y, et al. Cancer Lett, 2012, 315(1):28
[89] Kalluri R. J Clin Invest, 2016, 126(4):1208
[90] Gallo A, Tandon M, Alevizos I, et al. PloS One, 2012, 7(3):e30679
[91] Wang N, Wang L, Yang Y, et al. Biochem Biophys Res Commun, 2017, 493(3):1322
[92] Liu Q, Yu Z, Yuan S, et al. Oncotarget, 2017, 8(8):13048
[93] Lugli G, Cohen A M, Bennett D A, et al. PloS One, 2015, 10(10):e0139233
[94] Taylor D D, Gercel-Taylor C. Gynecol Oncol, 2008, 110(1):13
[95] Shi J. J Clin Med, 2016, 5(4):42
[96] Tanaka Y, Kamohara H, Kinoshita K, et al. Cancer, 2013, 119(6):1159
[97] Tsukamoto M, Iinuma H, Yagi T, et al. Oncology, 2017, 92(6):360
[98] Nilsson J, Skog J, Nordstrand A, et al. Br J Cancer, 2009, 100(10):1603
[99] Rodríguez M, Bajo-Santos C, Hessvik N P, et al. Mol Cancer, 2017, 16(1):156
[100] Khurana R, Ranches G, Schafferer S, et al. RNA, 2017, 23(2):142
[101] Skotland T, Ekroos K, Kauhanen D, et al. Eur J Cancer, 2017, 70:122
[102] Michael A, Bajracharya S D, Yuen P S, et al. Oral Dis, 2010, 16(1):34
[103] Wu C-X, Liu Z-F. J Invest Dermatol, 2018, 138(1):89
[104] Liu C-G, Song J, Zhang Y-Q, et al. Mol Med Rep, 2014, 10(5):2395
[105] Morse M A, Garst J, Osada T, et al. J Transl Med, 2005, 3(1):9
[106] Escudier B, Dorval T, Chaput N, et al. J Transl Med, 2005, 3(1):10
[107] Hood J L, San R S, Wickline S A. Cancer Res, 2011, 71(11):3792
[108] Pascucci L, Coccè V, Bonomi A, et al. J Control Release, 2014, 192:262
[109] Tian Y, Li S, Song J, et al. Biomaterials, 2014, 35(7):2383
[110] Haney M J, Klyachko N L, Zhao Y, et al. J Control Release, 2015, 207:18
[111] Yang J, Zhang X, Chen X, et al. Mol Ther-Nucl Acids, 2017, 7:278
[112] Zhuang X, Xiang X, Grizzle W, et al. Mol Ther, 2011, 19(10):1769