Abstract: A method was developed for the simultaneous determination of 11 benzimildazoles residue markers in chicken meat, liver and kidney samples by high performance liquid chromatography (HPLC). The chicken tissue sample was extracted with acetonitrile, defatted with n-hexane, and purified with an MCX solid-phase extraction cartridge. Chromatographic separation was performed on an Atlantis T₃ column with the gradient elution of methyl alcohol-1% (v/v) acetic acid aqueous solution as the mobile phases at a flow rate of 1.0 mL/min. The detection wavelength was set at 292 nm. Analytes were quantified by the external standard method. The linearities of the calibration curves were well in the range of 25-1000 μg/L, and the limit of quantification was 50 μg/kg. The recoveries at different spiked levels in all the three matrices ranged from 70.91% to 103.21%, and the relative standard deviations (RSDs) were between 1.48% and 10.08% (n=6). The developed method is characterized by an excellent purification effect, sensitivity and accuracy, and can be used for the determination of benzimidazoles residue markers in chicken tissue samples.

Key words: high performance liquid chromatography (HPLC), solid phase extraction(SPE), benzimidazoles residue markers, chicken tissue