Determination of ten cephalosporins in bee products by solid phase extraction-ultra-performance liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2019.07028

Abstract

Abstract: An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous quantitative determination of ten cephalosporins in bee products, including honey, royal jelly, and lyophilized royal jelly powder, has been established. The samples were extracted with an acetonitrile-water (80:20, v/v) solution. After purification by solid phase extraction using an Oasis PRIME HLB cartridge, the extract was blown to dryness under a stream of nitrogen gas and then re-dissolved in 1 mL 0.1% (v/v) formic acid solution and methanol (95:5, v/v). The samples were separated on an Acquity UPLC BEH C18 column, using a mixture of 0.1% (v/v) formic acid solution and methanol as the mobile phase under gradient elution. The analysis was carried out using a positive electrospray ion source in the multiple reaction monitoring mode. The matrix-matched external standard method was applied to quantitative analysis. Good linear relationships were obtained for the ten cephalosporins in certain concentration ranges, and the correlation coefficients were more than 0.999. The limits of detection and limits of quantification for the ten cephalosporins were in the ranges 0.15-1.5 μg/kg and 0.5-5 μg/kg, respectively. The recoveries for the analytes in the bee products were in the range of 75.0%to 89.8%, with relative standard deviations of 1.4% to 4.6%. This method is characterized by a short analysis time and is suitable for the determination of cephalosporins in different bee products by virtue of its simplicity and reliability.

Key words: solid phase extraction (SPE), ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), cephalosporins, bee products

CLC Number:

O658

ZHANG Hao, WU Yinliang, ZHANG Yiwen, XU Xiuqin, XU Feng. Determination of ten cephalosporins in bee products by solid phase extraction-ultra-performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2019, 37(12): 1314-1320.

References

[1] Zhang Y, Wu Y L. Journal of Instrumental Analysis, 2018, 37(11):1365 张艳, 吴银良. 分析测试学报, 2018, 37(11):1365
[2] Morelli B. J Pharm Biomed Anal, 2003, 32(2):257
[3] Peng F C, Shi C, Shi S L. China Pharmacy, 2003, 14(6):358 彭芳辰, 史岑, 史双来. 中国药房, 2003, 14(6):358
[4] Cui F Y, Zhang Z H, Li J H, et al. Journal of Food Safety and Quality, 2016, 7(2):575 崔凤云, 张朝晖, 李建辉, 等. 食品安全质量检测学报, 2016, 7(2):575
[5] Cheng J Q, Zhang F, Xu X L, et al. Chinese Journal of Chromatography, 2018, 36(8):809 程建桥, 张峰, 许秀丽, 等. 色谱, 2018, 36(8):809
[6] Gu B Q, Mei G M, Zhang X J, et al. Chinese Journal of Analytical Chemistry, 2017, 45(3):381 顾蓓乔, 梅光明, 张小军, 等. 分析化学, 2017, 45(3):381
[7] Li W Q, Shen H Y, Hong Y H, et al. J Chromatogr B, 2016, 1022:298
[8] Karageorgou E, Myridakis A, Stephanou E G, et al. J Sep Sci, 2013, 36(12):2020
[9] Baeza A N, Urraca J L, Chamorro R, et al. J Chromatogr A, 2016, 1474:121
[10] Kantiani L, Farre M, Grasesi Freixiedas J M, et al. Anal Bioanal Chem, 2010, 398(3):1195
[11] Kantiani L, Farre M, Grasesi Freixiedas J M, et al. J Chromatogr A, 2010, 1217(26):4247
[12] Perez-Burgos R, Greelak E M, Gokce G, et al. J Chromatogr B, 2010, 899:57
[13] Hou X L, Wu Y L, Lv Y, et al. J Chromatogr B, 2013, 931:6
[14] Yang X T, Tang X Y, Shen X X, et al. Chinese Journal of Analytical Chemistry, 2017, 45(7):1019 杨小体, 汤晓艳, 沈习习, 等. 分析化学, 2017, 45(7):1019
[15] Zhou Y, Zhang Y, Zhou Y P, et al. Chemical Research, 2016, 20(1):77 周昱, 张元, 周一平, 等. 化学研究, 2016, 20(1):77
[16] Gu B Q, Mei G M, Yu L, et al. Journal of Zhejiang Ocean University (Natural Science), 2015, 34(3):222 顾蓓乔, 梅光明, 喻亮, 等. 浙江海洋学院学报(自然科学版), 2015, 34(3):222
[17] Wang J F, Shi J L, Liu Y, et al. China Dairy Cattle, 2017(5):40 王建凤, 石佳丽, 刘艳, 等. 中国奶牛, 2017(5):40
[18] Zhang X G, Wang X, Dai C F, et al. Chinese Journal of Chromatography, 2018, 36(4):345 张小刚, 王霞, 戴春风, 等. 色谱, 2018, 36(4):345
[19] Legrand T, Vodovar D, Tournier N, et al. Antimicrob Agents Chemother, 2016, 60(8):4734
[20] Karageorgou E G, Samanidou V F, Papadoyannis I N. J Sep Sci, 2012, 35(19):2599
[21] Xu N, Sun H X, Zhang H, et al. China Dairy Industry, 2016, 44(2):50 许娜, 孙海新, 张慧, 等. 中国乳品工业, 2016, 44(2):50
[22] Chen C, Li Y, Wang X L, et al. Heilongjiang Animal Science and Veterinary Medicine, 2017(7):274 陈晨, 李阳, 王翔林, 等. 黑龙江畜牧兽医, 2017(7):274
[23] Tao H, Li W J, Chen W, et al. China Water and Wastewater, 2014, 30(10):96 陶辉, 李文君, 陈卫, 等. 中国给水排水, 2014, 30(10):96
[24] Zhang J, Zhao X R, Zhang Q, et al. The Food Industry, 2016, 37(2):269 张晶, 赵兴然, 张群, 等. 食品工业, 2016, 37(2):269
[25] Wang X Y, Qi K Z, Chen D D, et al. Jiangsu Journal of Agricultural Sciences, 2012, 28(1):193 汪雪雁, 祁克宗, 陈玎玎, 等. 江苏农业学报, 2012, 28(1):193
[26] Li M, Qiao W Y, Wang X L, et al. Chemical Research and Application, 2017, 29(5):604 李敏, 乔卫叶, 王秀玲, 等. 化学研究与应用, 2017, 29(5):604
[27] Morosini M I, Gareia-Castillo M, Tato M, et al. J Clin Microbiol, 2014, 52(5):1741
[28] Mo N, Zhang L J, Lü Z Y, et al. Journal of Dairy Science and Technology, 2018, 41(4):29 莫楠, 张立佳, 吕志勇, 等. 乳业科学与技术, 2018, 41(4):29
[29] Wu Q, He S H, Chen J, et al. Progress in Veterinary Medicine, 2015, 36(6):64 吴强, 何生虎, 陈娟, 等. 动物医学进展, 2015, 36(6):64
[30] Wang J F, Liu Y, Yang Y F, et al. Journal of Food Safety and Quality, 2015, 6(9):3380 王建凤, 刘艳, 杨一帆, 等. 食品安全质量检测学报, 2015, 6(9):3380
[31] Geng S T, Wang H H, Wang X L, et al. Anal Methods, 2015, 7(6):2413
[32] Bertram H C, Englsen S B, Bush H, et al. Meat Sci, 2004, 66(2):437