Automated hyphenation of in-tube boronate affinity solid-phase microextraction with high performance liquid chromatography for the determination of cis-diol compounds in tea beverages

doi:10.3724/SP.J.1123.2019.06020

Abstract

Abstract:

Automatic hyphenated analytical techniques hold great promise for reducing labour intensity, enhancing efficiency, and ensuring data reproducibility. In recent decade, Boronate affinity solid phase microextraction (BA-SPME) has emerged as a unique tool for the selective extraction of cis-diol-containing compounds. However, automatic hyphenation of SPME with high performance liquid chromatography (HPLC) has not yet been reported. Herein we report a new automated hyphenation of in-tube BA-SPME with HPLC for the determination of cis-diol compounds in tea beverages. The automated hyphenation was implemented through a flow-switch via the six-way valve of an autosampler. We prepared an in-tube SPME capillary, investigated the loading capacity of the coated column, characterized the morphology of the column, and investigated the factors influencing the separation. Finally, using the automatic hyphenated SPME-HPLC approach, we analyzed the cis-diol-containing compounds in three kinds of commercial tea beverages and investigated the effect of tea-making temperature on the content of the cis-diol-containing compounds in the prepared tea.

Key words: boronate affinity, liquid chromatography (LC), automated on-line hyphenation, solid phase microextraction (SPME), cis-diol compounds, tea beverage

WANG Xin, HE Jiangang, LUO Qi, LIU Zhen. Automated hyphenation of in-tube boronate affinity solid-phase microextraction with high performance liquid chromatography for the determination of cis-diol compounds in tea beverages[J]. Chinese Journal of Chromatography, 2020, 38(1): 137-142.

Figures/Tables 7

Fig. 1 Schematic diagram of automated on-line hyphenation of in-tube solid phase microextraction (SPME) with HPLC a. Extraction (Step 1): from the sample vial, the sample solution is repeatedly drawn into and ejected out of the in-tube SPME capillary; b. desorption and separation (Step 2): the in-tube SPME capillary is connected with the HPLC system.

Fig. 2 Schematic of the copolymerization reaction AIBN: 2, 2′-azobis(2-methylpropionitrile).

Fig. 3 Scanning electron microscope (SEM) images of the SPME capillary a. cross-sectional view of the capillary; b. enlarged image of the edge of the inner surface of the capillary.

Fig. 4 Effects of the flow rate of the mobile phase on the separation of cis-diol compounds extracted from tea beverage samples

Fig. 5 Comparison of the HPLC chromatograms with and without SPME

Fig. 6 Comparison of the chromatograms for different tea beverages

Fig. 7 Effects of tea-making temperature on the profile of cis-diol compounds in tea

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