Chinese Journal of Chromatography ›› 2020, Vol. 38 ›› Issue (9): 1013-1021.DOI: 10.3724/SP.J.1123.2020.02025
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WANG Fang1, WANG Song1, CONG Hailin1,2, YU Bing1,2,*()
Received:
2020-03-04
Online:
2020-09-08
Published:
2020-12-11
Contact:
YU Bing
Supported by:
WANG Fang, WANG Song, CONG Hailin, YU Bing. Analysis of metabolomics and proteomics based on capillary electrophoresis-mass spectrometry[J]. Chinese Journal of Chromatography, 2020, 38(9): 1013-1021.
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URL: https://www.chrom-china.com/EN/10.3724/SP.J.1123.2020.02025
Fig. 2 (a) Schematic of the sheathless CE-ESI-MS interface and (b) analysis of m/z of metabolites by CE-MS at different interfaces For Fig. 2a: 1. plastic plate; 2. electrodialysis membrane; 3. separation capillaries; 4. platinum electrodes; 5. buffer reservoirs. For Fig. 2b: black line, unsheathed; grey line, sheath flow. Reproduced from Ref. [30] with permission.
Fig. 3 Comparison of migration time and migration electrophoresis rate of a set of standard compounds on different analysis platforms Reproduced from Ref. [34] with permission.
Fig. 5 (a) Metabolic analysis of CE-MS acidic compounds in cation mode and (b) nucleotide profile after sample preconcentration Reproduced from Ref. [49] with permission.
Fig. 6 Analysis of protein mix by nano-LC-CZE-MS The mix contained RNase B (100 μg/mL), cytochrome c (80 μg/mL), lysozyme c (80 μg/mL), and myoglobin (50 μg/mL). First dimension (a): nano-LC chromatogram (214 nm detection wavelength) of the separation of different proteins. Second dimension (b): CZE-MS electropherogram obtained after a heart-cut of RNase B peak (transfer volume: 20 nL). Reproduced from Ref. [55] with permission.
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