|
|
Isolation and purification of flavones from Murraya exotica L. by high-speed counter-current chromatography
PENG Aiyi, QU Xuewei, LI Hui, GAO Lu, YU Bo, YANG Hong
2010, 28 (4):
383-387.
DOI: 10.3724/SP.J.1123.2010.00383
High-speed counter-current chromatography (HSCCC) was used to isolate and purify flavones from Murraya exotica L. The optimum separation conditions were as follows: A two-phase solvent system was petroleum ether-ethyl acetate-methanol-water (5:5:4.8:5, v/v/v/v). The lower phase as the mobile phase was operated at a flow rate of 2.0 mL/min, while the apparatus rotated at 800 r/min. Each time 200 mg of the sample was loaded. Under these conditions, 54.31 mg of recrystallized 5,7,3′,4′,5′-pentamethoxyflavone, 107.68 mg of 5-hydroxy-6,7,3′,4′-tetramethoxyflavone, 215.54 mg of 5-hydroxy-6,7,8,3′,4′-pentamethoxyflavone, and 84.36 mg of 5-hydroxy-6,7,8,3′,4′,5′-hexamethoxyflavone with their purities over 95% were successfully obtained from 4.0 g of the crude extract of Murraya exotica L. The four compounds were analyzed by high performance liquid chromatography (HPLC), and identified by mass spectrometry (MS), 1H-nuclear magnetic resonance (NMR) and 13C-NMR. The compound 5-hydroxy-6,7,3′,4′-tetramethoxyflavone was for the first time isolated and purified from Murraya exotica L.
|
