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Simultaneous determination of 57 residual volatile organic solvents in honey by headspace gas chromatography-mass spectrometry
LIU Yongming*, GE Na, WANG Fei, LI Jin, WU Yanping, HUANG Xuezhe, CAO Yanzhong
2012, 30 (08):
782-791.
DOI: 10.3724/SP.J.1123.2012.03050
A method was developed for the simultaneous determination of 57 residual volatile organic solvents (including several alkanes, aromatic hydrocarbons, alcohols, ketones, esters and ethers) in honey by headspace gas chromatography-mass spectrometry (HS-GC/MS). The honey sample was dissolved with water in a headspace vial, and the equilibration of the sample in the headspace vessel was achieved at 80 ℃ in 30 min. A DB-624 capillary chromatographic column (60 m×0.25 mm×1.40 μm) was used for the separation of 57 volatile organic solvents, and the analysis was performed by GC/MS. The external calibrations were used for the quantification. The linear ranges of the method were 0.005~0.2 μg for the alkanes, aromatic hydrocarbons and ethers, 0.05~2.0 μg for the esters, 0.5~20 μg for the ketones, 2.5~100 μg for the alcohols. The correlation coefficients were more than 0.996 for all the volatile organic solvents. The recoveries and the relative standard deviations were from 61.0% to 113.1% and 1.9% to 9.8%, respectively, at the spiked levels of 1.0~20 μg/kg for the alkanes, aromatic hydrocarbons and ethers, 10~200 μg/kg for the esters, 100~2000 μg/kg for the ketones, 500~10000 μg/kg for the alcohols. The limits of detection were 1.0 μg/kg for the alkanes, aromatic hydrocarbons and ethers, 10 μg/kg for the esters, 100 μg/kg for the ketones, 500 μg/kg for the alcohols. The method is simple, rapid, sensitive and accurate, and can be used for the simultaneous determination of residual volatile organic solvents in honey samples.
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