基于镜像酶正交酶切的蛋白质复合物规模化精准分析新方法
韩若楠1,2,#, 赵丽丽2,3,#, 安雨馨2,3, 梁振2, 赵群2,*(
), 张丽华2, 张玉奎1,2
), 张丽华2, 张玉奎1,2
Mirror cutting-assisted orthogonal digestion enabling large-scale and accurate protein complex characterization
HAN Ruonan1,2,#, ZHAO Lili2,3,#, AN Yuxin2,3, LIANG Zhen2, ZHAO Qun2,*(), ZHANG Lihua2, ZHANG Yukui1,2
), ZHANG Lihua2, ZHANG Yukui1,2
图2. LysargiNase与胰蛋白酶酶解样品的交联位点对及单一交联位点的互补性
Fig. 2. Complementarity of LysargiNase and trypsin digested cross-linked site pairs and single cross-linked sites
a. venn diagram of single cross-linked sites; b. venn diagram of the cross-linked site pairs; c. number of cross-linked site pairs of each single cross-linked site, digested with LysargiNase and trypsin.
